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csf 1  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec csf 1
    Csf 1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m+csf/Human+M-CSF%2C+premium+grade/pmc13691930-36-9-11
    Average 96 stars, based on 101 article reviews
    csf 1 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: A putative type IVB secretion system contributes to the pathogenicity of Yersinia pseudotuberculosis lineage 8 strains associated to the Far East scarlet-like fever
    Article Snippet: .. Collected cells were seeded at 1 × 106 cells/mL in petri dish (Greiner Bioone) in cell culture media supplemented with M-CSF (25 μg/mL, mouse M-CSF #130-101-704, Miltenyi Biotec) and antibiotics, and cultured at 37°C, 5% CO2 for 5 days. ..

    Article Title: Macrophage ferritin heavy chain/ α -synuclein regulatory axis modulates ferroptosis during kidney injury
    Article Snippet: .. BMDMs were cultured for 5 days in DMEM (Corning, 10-013-CV) with 15% FBS (R&D Systems, S11150 ), 1% penicillin-streptomycin (Gibco, 15140122), 1% MEM non-essential amino acids (Gibco, 11140050), and 0.3 μg/mL M-CSF (Miltenyi Biotec, 130-101-704). .. On day 6, the culture medium was replaced with DMEM containing 1% FBS for 2 hours, followed by treatment with either vehicle, mouse recombinant monomeric SNCA protein monomers (Proteos, RP-011), or fibrils at a dose of 1 μM (StressMarq, SPR-324).

    other:


    Isolation:

    Article Title: A MERTK-Targeting Antibody-Drug Conjugate Selectively Depletes M2 Tumor-Associated Macrophages and MERTK-Expressing Cancer Cells
    Article Snippet: Luminescence was read on a GloMax Explorer (Promega, GM3500). .. Human M2 macrophages were differentiated by culturing 2.5× 10 5 monocytes isolated from PBMCs as described above in 24-well plates in RPMI 1640 medium (Gibco, 11875093) containing 10% FBS, penicillin, streptomycin and M-CSF (50 ng/mL; Miltenyi, 130-096-485), and incubated at 37°C with 5% CO 2 for 6 days. .. MERTK-KO SKMel5 were labeled with CFSE dye with CFSE Cell Division Tracker Kit (Biolegend, 423801) according to the manufacturer’s instruction.

    Article Title: A scalable human-zebrafish xenotransplantation model reveals gastrosome-mediated processing of dying neurons by human microglia.
    Article Snippet: Differentiation of iPSC-microglia (iMGL) from iPSCs Human iPSC were differentiated to hemotopoietic progenitor cells (iHPC) using the Hematopoietic Kit (STEMCELL Technologies) and then used to further differentiate mature induced microglia-like cells (iMGL) using the Microglia Differentiation and Microglia Maturation kits (STEMCELL Technologies) by following the manufacturer guidelines. .. Co-culture of isolated EMPs with cortical organoids was performed at 42 days of forebrain organoid differentiation using 100,000 cells in the aboveindicated organoid differentiation media supplemented with 25 ng ml-1 M-CSF (Miltenyi), 100 ng ml-1 IL34 (Miltenyi) and 50 ng ml-1 TGFβ1 (Miltenyi). .. Human iPSC colonies were detached before reaching confluency with collagenase Type IV (Gibco) and transferred to an Ultra-Low attachment 10-cm plate (Corning Costar) containing 10 ml hPSC medium consisting of DMEM:F12 (Invitrogen), 20% Knockout Serum Replacement (Gibco), 1× Non-essential Amino Acids (Invitrogen), 1× 2-mercaptoethanol (Gibco), 1× GlutaMAX (Invitrogen), 10 ng ml–1 FGF-2 (Peprotech) and ROCK inhibitor Y27632 (10 μM).

    Incubation:

    Article Title: A MERTK-Targeting Antibody-Drug Conjugate Selectively Depletes M2 Tumor-Associated Macrophages and MERTK-Expressing Cancer Cells
    Article Snippet: Luminescence was read on a GloMax Explorer (Promega, GM3500). .. Human M2 macrophages were differentiated by culturing 2.5× 10 5 monocytes isolated from PBMCs as described above in 24-well plates in RPMI 1640 medium (Gibco, 11875093) containing 10% FBS, penicillin, streptomycin and M-CSF (50 ng/mL; Miltenyi, 130-096-485), and incubated at 37°C with 5% CO 2 for 6 days. .. MERTK-KO SKMel5 were labeled with CFSE dye with CFSE Cell Division Tracker Kit (Biolegend, 423801) according to the manufacturer’s instruction.



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